Mitochondrial NADH Fluorescence Is Enhanced by Complex I Binding
نویسندگان
چکیده
منابع مشابه
Investigation of NADH Binding, Hydride Transfer, and NAD+ Dissociation during NADH Oxidation by Mitochondrial Complex I Using Modified Nicotinamide Nucleotides
NADH:ubiquinone oxidoreductase (complex I) is a complicated respiratory enzyme that conserves the energy from NADH oxidation, coupled to ubiquinone reduction, as a proton motive force across the mitochondrial inner membrane. During catalysis, NADH oxidation by a flavin mononucleotide is followed by electron transfer to a chain of iron-sulfur clusters. Alternatively, the flavin may be reoxidized...
متن کاملMitochondrial Complex I Is an Essential Player in LPS-Induced Preconditioning in Differentiated PC12 Cells
Preconditioning (PC) as a protective strategy against noxious insults can decline cell death and apoptosis. It has been approved that mitochondria play a key role in PC mechanism. The critical role of complex I (CI) in oxidative phosphorylation machinery and intracellular ROS production, particularly in the brain, accentuates its possible role in PC-induced neuroprotection. Here, differentiated...
متن کاملMitochondrial Complex I Is an Essential Player in LPS-Induced Preconditioning in Differentiated PC12 Cells
Preconditioning (PC) as a protective strategy against noxious insults can decline cell death and apoptosis. It has been approved that mitochondria play a key role in PC mechanism. The critical role of complex I (CI) in oxidative phosphorylation machinery and intracellular ROS production, particularly in the brain, accentuates its possible role in PC-induced neuroprotection. Here, differentiated...
متن کاملEvidence for Two Sites of Superoxide Production by Mitochondrial Nadh-q Oxidoreductase (complex I)*
EVIDENCE FOR TWO SITES OF SUPEROXIDE PRODUCTION BY MITOCHONDRIAL NADH-Q OXIDOREDUCTASE (COMPLEX I)* Jason R. Treberg, Casey L. Quinlan and Martin D. Brand Buck Institute for Research on Aging, Novato, CA, 94945, USA Running head: Two sites of superoxide production by complex I Address correspondence to: Jason R. Treberg, 8001 Redwood Blvd., Novato, CA, 94945. FAX: 415 209 2232. Email: jtreberg@...
متن کاملLife without complex I: proteome analyses of an Arabidopsis mutant lacking the mitochondrial NADH dehydrogenase complex
The mitochondrial NADH dehydrogenase complex (complex I) is of particular importance for the respiratory chain in mitochondria. It is the major electron entry site for the mitochondrial electron transport chain (mETC) and therefore of great significance for mitochondrial ATP generation. We recently described an Arabidopsis thaliana double-mutant lacking the genes encoding the carbonic anhydrase...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemistry
سال: 2008
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi800307y